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(480)727-0435

Nagaraj Vinay Janthakahalli, PhD

Research Scientist

The Biodesign Institute, Bioelectronics and Biosensors

Bio

RESEARCH INTERESTS

Novel biological applications of piezoelectric liquid dispensing technology including microarray-based analysis of cellular function by siRNA transfection; sensor platforms for detection of cells and biomarkers; discovery of cancer therapeutics and high throughput proteomics and glycomics.

JOURNAL PUBLICATIONS

  1. Nagaraj VJ, Eaton S, Wiktor P (2010). NanoProbeArrays for the Analysis of Ultra-Low-Volume Protein Samples Using Piezoelectric Liquid Dispensing Technology. Journal of the Association for Laboratory Automation doi:10.1016/j.jala.2010.07.005
  2. Nagaraj VJ, Aithal S, Eaton S, Bothara M, Wiktor P, Prasad S (2010). NanoMonitor: Miniature Electronic Biosensor for Glycan Biomarker Detection. Nanomedicine: 5(3): 369-378(10)
  3. Ritsema T, Brodmann D, Diks SH, Bos CL, Nagaraj V, Pieterse CMJ, Boller T, Wiemken A, Peppelenbosch MP (2009). Are small GTPases signal hubs in sugar-mediated induction of fructan biosynthesis. PLoS One. Aug 12;4(8): e6605.
  4. Kilcoyne M, Shah M, Gerlach JQ, Bhavanandan VP, Nagaraj VJ, Smith AD, Fujiyama K, Sommer U, Costello CE, Olszewski N, Joshi L (2009) O-glycosylation of protein subpopulations in alcohol-extracted rice proteins. Journal of Plant Physiology. Feb 15;166(3):219-32.
  5. Nagaraj VJ, Eaton S, Thirstrup D, Wiktor P (2008). Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis. Biochem Biophys Res Commun. 2008 Oct 31;375(4):526-30.
  6. Martínez-Noël G, Nagaraj VJ, Calo G, Wiemken A, Pontis H (2007). Sucrose regulated expression of a Ca2 -dependent protein kinase (TaCDPK1) gene in excised leaves of wheat. Plant Physiology and Biochemistry 45(6-7):410-419
  7. Nagesha N, Gowda PHR, Madhusudana SN, Joshi L, Nagaraj VJ, Kilcoyne M, Devaiah BN, Madhuvanthi R, Vanikulkarni, Saraswathi S, Dinesh A,N, Gowda TKS, Mehamooda K (2007). Genetic transformation of cantaloupe melon (Cucumis melo L.) with the rabies virus glycoprotein gene (PRGSpRgp) and immunization studies in mice. The Journal of Horticultural Science and Biotechnology 82(3):383-386
  8. Martínez-Noël G, Togneti J, Nagaraj VJ, Wiemken A, Pontis H (2006). Calcium is essential for fructan synthesis induction mediated by sucrose in wheat. Planta 225(1):183-91.
  9. Van Riet L, Nagaraj VJ, Clerens S, Van den Ende W, Wiemken A, Van Laere A (2006). Purification, cloning and functional characterization of a fructan 6-exohydrolase (6-FEH) from wheat (Triticum aestivum L.).Journal of Experimental Botany 57(1):213-223.
  10. Nagaraj VJ, Galati V, Lüscher M, Boller T, Wiemken A (2005). Cloning and functional characterization of a cDNA encoding barley soluble acid invertase (HvINV1). Plant Science 168: 249-258.
  11. Tran N, Baral C, Nagaraj VJ and Joshi L (2005). Knowledge-Based Framework for Hypothesis Formation in Biochemical Networks. Bioinformatics. 2005 Sep 1;21 Suppl 2:ii213-ii219.
  12. Elfstrand M, Feddermann N, Ineichen K, Nagaraj VJ, Wiemken A, Boller T, Salzer P (2005). Ectopic expression of the arbuscule-specific gene Mtchit 3-3 stimulates germination of glomalean fungi. New Phytologist 167 (2), 557-570.
  13. Tran N, Baral C, Nagaraj VJ and Joshi L (2005). Knowledge-Based Integrative Framework for Hypothesis Formation in Biochemical Networks. Lecture Notes in Computer Science 3615: 121-136.
  14. Nagaraj VJ, Altenbach D, Galati V, Lüscher M, Meyer AD, Boller T, Wiemken A (2004). Distinct regulation of sucrose:sucrose-1-fructosyltransferase (1-SST) and sucrose:fructan-6-fructosyltransferase (6-SFT), the key enzymes of fructan synthesis in barley leaves: 1-SST as the pacemaker. New Phytologist 161(3): 735-748.
  15. Nagaraj VJ, Riedl R, Boller T, Wiemken A, Meyer AD (2001) Light and sugar regulation of the barley sucrose:fructan 6- fructosyltransferase promoter. Journal of Plant Physiology 158: 1601-1607.

 

NEUCLOTIDE SEQUENCE PUBLICATIONS IN EMBL/GENBANK/DDBJ DATABASES

 

  1. AJ306962: Hordeum vulgare sucrose:fructan 6-fructosyltransferase gene promoter
  2. AJ585414: Festuca arundinacea sucrose:sucrose 1-fructosyltransferase gene promoter
  3. AJ605333: Hordeum vulgare mRNA for fructan 1-exohydrolase precursor gene
  4. AM075205: Triticum aestivum mRNA for fructan 6-exohydrolase (6-FEH)
  5. AJ567377: Hordeum vulgare mRNA for sucrose:sucrose 1-fructosyltransferase
  6. AJ605333: Hordeum vulgare mRNA for soluble acid invertase (HvSAI)
  7. AJ621356: Triticum aestivum mRNA for putative calcium-dependent protein kinase
  8. AY238969: Medicago truncatula chitinase (chitIII-3) gene, complete cDNA
  9. AY238970: Medicago truncatula chitinase (chitIII-4) gene, complete cDNA
  10. AY294484: Medicago truncatula chitinase (chitIII-1) gene, complete cDNA

 

CONFERENCE ABSTRACT PUBLICATIONS

  1. MRS 2009: Annual Meeting of the Materials Research Society, Boston, Massachusetts, USA (November-December 2009). Enhanced Electrochemical Detection of Protein Glycosylation using Biogenic Silica Based Nanopores. Aithal S, Chatterjee S, Lin K, Ramakrishna B L, Nagaraj VJ, Wiktor P and Prasad S.  Proceedings of the Materials Research Society 2009 Fall Meeting, Volumes 1194-1241.
  2. MRS 2009: Annual Meeting of the Materials Research Society, Boston, Massachusetts, USA (November-December 2009). Bothara M, Nagaraj VJ, Eaton S, Aithal S, Chatterjee G, Wiktor P, and Prasad S. Nanomonitor technology for Glycosylation Analysis. Proceedings of the Materials Research Society 2009 Fall Meeting, Volumes 1194-1241.
  3. AAAS PD 2009: Annual Meeting of The American Association for the Advancement of Science, Pacific Division, San Francisco, California, USA (August 2009). Chatterjee G, Nagaraj VJ, Wiktor P, Eaton S and Prasad S. Nanomonitor Technology for Glycosylation Analysis. Proceedings of the Pacific Division, AAAS 28(1):64 [abstract #36].
  4. Glycobiology 2008: Annual Conference of the Society for Glycobiology, Fort Worth, Texas, USA (November 2008). Nagaraj VJ, Eaton S, Thirstrup D, Wiktor P. Glycosylation Analysis by Piezoelectric Printing and Probing of Lectin NanoProbeArrays. Glycobiology 18 (11): 941-942. (Invited speaker at this conference)
  5. AACR 2008: Annual Meeting of the American Association for Cancer Research, San Diego, California, USA (April 2008). Agrawal P, Gu J, Hurth C, Yang J, Nagaraj VJ, Zenhausern F and Berens M. Imprinting Soft Biological Gels Below One Micron Feature Size for 3-D Cell Culture and Microenvironment. Published Online at the Proceedings of the American Association for Cancer Research 2008.
  6. AAAS 2007: Annual Meeting of The American Association for the Advancement of Science, St. Louis, Missouri, USA (Feb 2007). Riblett A, Chow K, Nagaraj VJ, Shah M, Joshi L. Cell capture using CLIO nanoparticles conjugated to glycan binding peptides. AAAS 2007 Book of Abstracts: A330.
  7. Glycobiology 2006: Annual Conference of the Society for Glycobiology, Los Angeles, California, USA (Nov 2006). Boltz K, Nagaraj VJ, Svarovsky S, Lake D, Stafford P, Joshi L. High Throughput Technology for the Identification and Characterization of Glycan Binding Peptides. Glycobiology (November 2006  issue): 1155-1156.
  8. AAAS 2006: Annual Meeting of The American Association for the Advancement of Science, St. Louis, Missouri, USA (Feb 2006). Chow K, Nagaraj VJ, Shah M Joshi L. Localization of Arabidopsis thaliana sialyltransferases heterologously expressed in CHO cells. AAAS 2006 Book of Abstracts: A162.
  9. ECCB 2005: The 4th European Conference on Computational Biology, Madrid, Spain (Sept  2005). Tran N, Baral C, Nagaraj VJ, Joshi L. Knowledge-based framework for hypothesis formation in biochemical networks. ECCB/JBI 2005: 219
  10. DILS 2005: The 2nd International Workshop on Data Integration in the Life Sciences, San Diego, USA, (July 2005). Tran N, Baral C, Nagaraj VJ, Joshi L. Knowledge-based integrative framework for hypothesis formation in biochemical networks. DILS 2005: 121-136
  11. Glycobiology 2004: Annual Conference of the Society for Glycobiology, Honolulu, Hawaii, USA (Nov 2004). Joshi L, Shah M, Daskalova S, Nagaraj VJ, Prasanna C, Gerlach J, Smith AG. Plant Sialobiology. Glycobiology 14 (11): 1060-1060.
  12. Fructan 2004: Fifth International Fructan Symposium Havana, Cuba (Dec 2004). Ritsema T, Tuynman J, Diks S, Bos C, Brodmann D, Nagaraj VJ, Peppelenbosch M, Boller T, Wiemken A. The promoter of sucrose:fructan 6-fructosyl-transferase (SFT) and its regulation by sucrose. Fructan 2004 Book of Abstracts: 69.
  13. GRC 2002: Gordon Research Conference on CO2 Fixation and Metabolism in Green Plants, Massachusetts, USA (Aug 2002). Nagaraj VJ, Wiemken A. Regulation of Fructan Metabolism in Barley Leaves.
  14. Fructan 2000: Fourth International Fructan Symposium, Arolla, Switzerland (Aug 2000). Nagaraj VJ, Wiemken A, Meyer AD. Identification and Characterization of the Barley 6-SFT Gene Promoter. Fructan 2000 Book of Abstracts: 14. (Invited speaker at this conference)
  15. NRBN 1999: Seventh National Rice Biotechnology Network Meeting, Bangalore, India (October 1999). Nagaraj V J and Morawala-Patell V (1999) A comparative study of the gene expression at the mRNA level during abiotic stress in the seedlings of Indica Rice varieties IR-64 and RASI using heterologous cDNA probes. NRBN ’99 Book of Abstracts.